Last edited by Nikokinos
Friday, July 31, 2020 | History

8 edition of Molecular Beacons found in the catalog.

Molecular Beacons

Signalling Nucleic Acid Probes, Methods, and Protocols (Methods in Molecular Biology)

  • 219 Want to read
  • 40 Currently reading

Published by Humana Press .
Written in English

    Subjects:
  • Medical diagnosis,
  • Molecular biology,
  • Science,
  • Science/Mathematics,
  • Biotechnology,
  • Life Sciences - Genetics & Genomics,
  • Life Sciences - Zoology - General,
  • DNA,
  • Fluorescence,
  • Genomics,
  • Microarrays,
  • Probes,
  • Ribozyme,
  • Science / Genetics

  • Edition Notes

    ContributionsAndreas Marx (Editor), Oliver Seitz (Editor)
    The Physical Object
    FormatHardcover
    Number of Pages274
    ID Numbers
    Open LibraryOL8828627M
    ISBN 101588297004
    ISBN 109781588297006

    The chapter describes a detection method that is based on the sequence-dependent hybridization of fluorogenic reporter molecules called “molecular beacons’’ and its applications. Molecular beacon probes represent a new class of oligonucleotides that can hybridize and report the presence of specific nucleic acids in homogeneous solutions. Weihong Tan demonstrates that molecular beacons are not limited to the detection of nucleic acids but can also be used to report on DNA-protein interactions. Daniel Summerer shows how molecular beacon technology can be fashioned into a new tool that facilitates the molecular enzymology of .

    Molecular beacon. Molecular beacons are stem-loop structures where the ends of the oligonucleotide are self-complementary and the center portion of the molecule is complementary to the target sequence (A). Self-annealing of the ends brings the fluorophore (F) and quencher (Q) dyes into close proximity, and the molecule will not fluoresce. The objective of this study is to visualize the ability of cell proliferation based on molecular beacons (MB). Two types of MB to detect messenger RNA (mRNA) were used. One is a Ki67 MB of a target.

    This has invoked the recent development of molecular imaging strategies capable of illuminating the distribution and dynamics of RNA molecules in living cells. In this review, we describe a class of molecular imaging probes known as molecular beacons (MBs), which have increasingly become the probe of choice for imaging RNA in living cells. A beacon is an intentionally conspicuous device designed to attract attention to a specific location.A common example is the lighthouse, which provides a fixed location that can be used to navigate around obstacles or into modern examples include a variety of radio beacons that can be read on radio direction finders in all weather, and radar transponders that appear on radar displays.


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Molecular Beacons Download PDF EPUB FB2

Molecular beacons are probe molecules that exhibit a characteristic stem-loop structure through which the 5′ and 3′ ends are maintained in close proximity (Fig.

).In a manner similar to the TaqMan probes described above, fluorescence from a fluorochrome at one end. Molecular Beacons explains working principle of molecular beacons, discusses their design, synthesis, purification and characterization, explores their thermodynamic and kinetic properties, and more importantly, reviews their in vivo and in vitro applications with the emphasis on the design and modification of molecular beacons for in vivo mRNA Molecular Beacons book : Chaoyong James Yang.

Molecular Beacons Introduction to Molecular Beacons. Molecular beacons are single stranded hairpin shaped oligonucleotide probes. In the presence of the target sequence, they unfold, bind and fluoresce. The molecular beacon chemistry is one of the chemistries used to carry out a real time experiment.

Molecular Beacons explains working principle of molecular beacons, discusses their design, synthesis, purification and characterization, explores their thermodynamic and kinetic properties, and more importantly, reviews their in vivo and in vitro applications with the emphasis on the design and modification of molecular beacons for in vivo mRNA imaging applications.

A molecular beacon is the hairpin-shaped single-stranded structured probe which is highly sequence-specific and sensitive. The Molecular Beacons. are used in the real-time PCR assay for the quantification of RNA or DNA present into the sample. Molecular beacons are increasingly being used in many applications involving nucleic acid detection and quantification.

The stem–loop structure of molecular beacons provides a competing reaction for probe–target hybridization that serves to increase probe specificity, which is particularly useful when single-base discrimination is desired. Molecular beacons (MBs) have shown fascinating applications in many biological fields.

However, exploration of cost-effective, sensitive, stable and efficient MB for in situ live cell- based assay has still room for improvement. In this regards, we have developed a novel MB Molecular Beacons book bears a spherical graphite nanoparticle (GN) as a fluorescent quencher.

Book January w Reads How we measure 'reads' A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a.

The book is written with a preference for breadth over depth, beginning with several chapters to cover the basics for students with a non-molecular background in biology and continuing on in survey fashion to the topics of genomics research, RNA and protein function, gene expression, subcellular life-forms, and DNA alteration."--Reference & Research Book News, October Introduction.

When molecular beacon hybridization probes [] are used to detect different target sequences in traditional PCR assays, each molecular beacon is labeled with a single uniquely colored fluorophore [], and the number of different target sequences that can be identified is limited to the number of differently colored fluorophores that the PCR instrument can distinguish [3–5].

Abstract We have prepared a novel optical fiber evanescent wave DNA biosensor using a newly developed molecular beacon DNA probe. The molecular beacons (MB) are oligonucleotide probes that become fluorescent upon hybridization with target DNA/RNA molecules.

The book first offers an introduction to the basic principles of fluorescence and then describes applications of fluorogenic probes in real-time PCR, which currently is the gold standard for quantitative DNA and RNA analysis.

Coverage extends the potential of realtime as well as advocates simplifications of the probe : $ Molecular Beacons explains working principle of molecular beacons, discusses their design, synthesis, purification and characterization, explores their thermodynamic and kinetic properties, and more importantly, reviews their in vivo and in vitro applications with the emphasis on the design and modification of molecular beacons for in vivo mRNA imaging applications.

This book is designed to. Guided by experts in the field, Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols, provides easy-to-follow experiments, commences with an introduction to the basic principles of fluorescence, and shows a diverse set of instructive examples, from probe design to applications in clinical settings, molecular beacon technology, the identification of transgenic mice, along with the Price: $ We have proposed a novel strategy for miRNA detection through enzyme-free signal amplification by self-circulation of the hybridization between the miRNAs and molecular beacon (MB) circuits.

Unlike general MB-based miRNA detection based on the one-to-one (1:. Upon the addition of Exo III, the molecular beacon probe was digested into mono- or short oligonucleotides by the exonucleases and failed to form fluorescent CuNPs. As a result, the fluorescence intensity of CuNPs decreased. This method exhibited a very low detection limit of U mL −1 for Exo III.

The designed molecular beacon probe can. Molecular beacons are nucleic acid hybridization probes. They are designed to bind to target DNA sequences in regions, such as the rpoB, where resistance mutations are known to occur.

Molecular beacons will fluoresce only when bound to their targets so that a mutation – even a single nucleotide substitution – will prevent fluorescence.

Marras SAE, Tyagi S, Antson D, Kramer FR () Color-coded molecular beacons for multiplex PCR screening assays. PLoS ONE e Vargas DY, Marras SAE, Tyagi S, Kramer FR () Suppression of Wild-Type Amplification by Selectivity Enhancing Agents in PCR Assays that Utilize SuperSelective Primers for the Detection of Rare Somatic Mutations.

View all on PubMed. Marras SAE, Tyagi S, Antson D, Kramer FR () Color-coded molecular beacons for multiplex PCR screening One e Vargas DY, Marras SAE, Tyagi S, Kramer FR () Suppression of Wild-Type Amplification by Selectivity Enhancing Agents in PCR Assays that Utilize SuperSelective Primers for the Detection of Rare Somatic Mutations.

High-sensitive and high-affinity methods to measure gene expression inside living cells have proven to be invaluable in regards to understanding fundamental processes such as cell differentiation, reprogramming, regeneration and cancer genesis.

One tool for transcription visualization on single cell level is molecular beacons (MBs). Molecular Methods In Diagnosis is a important topic to be understood by laboratory technology personal for implementation of emerging technologies for the grow.I understand how molecular beacon and Taqman probe work.

But just wondering why the Taq polymerase won't cleave the molecular beacon probes during extension considering its 3'-5' exonuclease activity.

Of the clones containing CA and GATA repeats, we were able to identify clones (CA, n = 99; GATA, n = 30) by using molecular beacons and only clones (CA, n = 92; GATA, n = 29) by conventional PCR amplification. As anticipated, 55 clones that contained sequences other than CA or GATA failed molecular beacon detection.